IL4 Receptor Protein (Transcript Variant 1)

N° du produit ABIN2713396

Cette protéine Recombinant IL4 Receptor est exprimée dans HEK-293 Cells.

Aperçu rapide pour IL4 Receptor Protein (Transcript Variant 1) (ABIN2713396)

Antigène: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Application: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Pureté: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Détail du produit

Attributs du protein: Transcript Variant 1

Specificité: Optimal preservation of protein structure, post-translational modifications and functions.

Attributs du produit:

• Recombinant human CD124 / IL4R (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

niveau d'endotoxine: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is less than or equal to 5.0 ng/ml (in the presence of 0.5 ng/ml of IL-4), corresponding to a specific activity of > 2 x 10^5 units/mg.

Information d'application

Indications d'application: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Stockage

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Conseil sur la manipulation: Resuspend the protein in the desired concentration in proper buffer

Stock: -80 °C

Stockage commentaire: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Détail du antigène

Antigène: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Autre désignation: Cd124,il4r

Sujet: This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.

Poids moléculaire: 24 kDa

NCBI Accession: NP_000409

Pathways: Signalistation JAK/STAT, Positive Regulation of Immune Effector Process